BOD Water Analysis

Biochemical Oxygen Demand 

What is Biochemical Oxygen Demand (BOD)?  

Biochemical Oxygen Demand (BOD) refers to the amount of dissolved oxygen required by microorganisms to decompose organic matter in water, serving as a measure of pollution severity. BOD measurements gauge the decomposition of organic substances in water, reflecting the demand for oxygen. It is a widely utilized parameter for assessing the level of organic pollution in water bodies. High BOD values indicate the presence of substantial amounts of biodegradable organic matter, such as sewage, wastewater, or industrial effluents. Monitoring BOD enables the evaluation of overall water quality and the potential impacts on aquatic ecosystems.

BOD water analysis is crucial for evaluating the efficiency of wastewater treatment processes. By measuring BOD in both influent (raw) and effluent (treated) wastewater, operators can assess the effectiveness of organic pollutant removal during treatment. Monitoring BOD assists in optimizing treatment operations and ensuring compliance with regulatory standards.

Elevated BOD levels in water bodies can result in oxygen depletion and harmful conditions for aquatic life. Microorganisms consume dissolved oxygen while decomposing organic matter, thereby reducing oxygen availability for other organisms. Measuring BOD enables environmental scientists and regulators to assess the potential impact of organic pollution on the health of aquatic ecosystems and take necessary mitigation measures.

BOD measurements can assist in identifying sources of organic pollution in water bodies. By analyzing BOD profiles at different locations or over time, scientists and environmental managers can track the origin and extent of pollution, helping identify point sources, non-point sources, or changes in pollutant levels.

And finally, BOD data plays a crucial role in scientific research, hydrological studies, and water resource management. Understanding the organic pollution load, trends, and variations in BOD levels helps in formulating effective strategies for water quality improvement, pollution prevention, and sustainable management of water resources.

  • BOD Water Analysis

    WHAT are some standard methods for determining BOD?

    Biochemical Oxygen Demand (BOD) is a common parameter used to measure the amount of oxygen consumed by microorganisms during the biological degradation of organic matter in water. BOD analysis provides information about the organic pollution levels and the potential impact on aquatic ecosystems. Several standard methods are commonly used for determining BOD in water. Here are two widely recognized techniques:

    • Dilution Method (Standard Method): The dilution method, also known as the standard method, is one of the most widely used techniques for BOD determination. It involves incubating a diluted water sample under controlled laboratory conditions to simulate the natural degradation process. The key steps involved in the dilution method are as follows:
    • Sample Dilution: The water sample is diluted with a known volume of dilution water, usually distilled or deionized water, to achieve a suitable BOD range for accurate measurement.
    • Seed Addition: A seed or inoculum is added to the diluted sample. The seed typically consists of a mixture of microorganisms obtained from a healthy wastewater treatment plant or a standardized seed source.
    • Incubation: The diluted sample with the seed is placed in an airtight container, such as a BOD bottle, and incubated in the dark at a specified temperature (typically 20°C) for a fixed duration (commonly 5 days).
  • BOD Water Analysis

    WHAT is the Respirometric Measurement?

    Respirometric measurement is a technique used to determine the rate of oxygen consumption or uptake by microorganisms during the degradation of organic matter. It is commonly employed in various environmental and biological applications, including the determination of Biochemical Oxygen Demand (BOD) in water samples.

    The respirometric method involves the measurement of changes in dissolved oxygen (DO) concentration over time as an indicator of microbial activity. Here’s a general overview of the respirometric measurement method:

    • Sample Preparation: The water sample is collected and prepared for analysis. Depending on the specific application, the sample may require filtration or pretreatment to remove particulate matter or interfering substances.
    • Respirometric Setup: The prepared sample is added to a respirometer chamber or vessel, which typically consists of a closed system or a sealed container. The chamber is equipped with a sensitive DO probe that continuously monitors the DO concentration in the sample.
    • Oxygen Consumption Measurement: Once the sample is added to the respirometer chamber, the oxygen concentration is measured at regular intervals over a specific duration. The respirometer system may include controls for maintaining constant temperature, agitation, and other relevant parameters.
    • Data Analysis: The measured DO concentrations are recorded and used for data analysis. The change in DO concentration over time indicates the rate of oxygen consumption by microorganisms in the sample. This oxygen consumption rate reflects the microbial activity and degradation of organic matter present in the sample.
    • BOD Calculation: The respirometric data obtained from the measurement are analyzed and used to calculate the BOD value. The BOD value is typically expressed as the amount of oxygen consumed per unit volume of the sample (e.g., milligrams of oxygen per liter of sample).
  • BOD Water Analysis


    Biochemical Oxygen Demand (BOD) in water arises from the presence of organic substances that can be degraded by microorganisms. These organic substances can originate from various sources. Here are some common sources of BOD in water:

    • Domestic Wastewater: The discharge of wastewater from residential areas, including households, apartments, and other dwellings, contributes a significant amount of organic matter to water bodies. This wastewater contains organic compounds from human activities such as washing, bathing, and toilet usage.
    • Industrial Effluents: Industries that produce or process organic materials, such as food processing, pulp and paper mills, breweries, and textile factories, can release effluents containing high levels of organic matter. Industrial wastewater can contain organic compounds derived from production processes, cleaning operations, or product residues.
    • Agricultural Runoff: Agricultural activities, including crop cultivation, animal farming, and irrigation practices, can contribute to the organic load in water bodies. Runoff from agricultural areas may contain organic matter in the form of fertilizers, pesticides, animal waste, and decaying plant material.
    • Stormwater Runoff: Urban areas and paved surfaces generate stormwater runoff that can transport organic matter accumulated on streets, parking lots, and rooftops. This runoff can carry leaves, grass clippings, soil particles, and other organic debris into water bodies, contributing to BOD.
    • Natural Organic Matter: Organic matter occurs naturally in water bodies due to the decomposition of vegetation, algae, and other biological materials. Leaves, twigs, and other plant debris that fall into rivers, lakes, or ponds can increase the organic load and subsequent BOD levels.
    • Sewage Discharges: Improperly treated or untreated sewage discharges directly into water bodies introduce a significant amount of organic matter, contributing to high BOD levels. This can occur in regions with inadequate sanitation infrastructure or during sewage system malfunctions.
  • BOD Water Analysis


    Water toxicity refers to the harmful or detrimental effects of water or waterborne substances on living organisms, including plants, animals, and humans. It is a measure of the potential of water to cause adverse effects on organisms when they are exposed to it.

    Water toxicity can result from various factors, including chemical pollutants, heavy metals, pesticides, industrial contaminants, and naturally occurring substances. These substances can enter water bodies through direct discharges, runoff from agricultural or industrial activities, accidental spills, or natural processes such as erosion.

    Toxicity in water can have significant ecological consequences, impacting aquatic organisms, biodiversity, and the overall health of ecosystems. It can also pose risks to human health when contaminated water is used for drinking, recreational activities, or food production.

    The severity of water toxicity depends on several factors, including the concentration and persistence of the toxic substances, the exposure duration, and the sensitivity of the organisms involved. Different species exhibit varying levels of sensitivity to different toxicants.

    Water toxicity is typically evaluated through toxicological testing, which involves exposing organisms to water samples or specific chemicals under controlled laboratory conditions. These tests measure various endpoints, such as mortality, reproductive impairment, growth inhibition, behavioral changes, or biochemical markers of toxicity.

  • BOD Water Analysis


    The Nitrification Respiration Inhibition Test is a laboratory method used to assess the potential toxicity of substances to nitrifying bacteria. Nitrification is a crucial biological process in wastewater treatment systems and natural environments, where specific bacteria convert ammonia (NH3) to nitrite (NO2-) and subsequently to nitrate (NO3-).

    The Nitrification Respiration Inhibition Test is conducted by exposing nitrifying bacteria to different concentrations of a test substance and evaluating its impact on the respiration rate of the bacteria. The test measures the inhibition of oxygen consumption by the nitrifying bacteria as an indicator of potential toxicity to these organisms.

    Here’s a general overview of the NRIT procedure:

    • Test Organisms: The NRIT typically uses two species of nitrifying bacteria: Nitrosomonas spp. for ammonia oxidation and Nitrobacter spp. for nitrite oxidation. These bacteria are commonly obtained from laboratory cultures or wastewater treatment plant biomass.
    • Test Substance Preparation: The test substance, which can be a chemical or a complex mixture, is prepared at different concentrations. It is important to include a range of concentrations, including a control without the test substance, to establish dose-response relationships and determine the concentration at which toxicity occurs.
    • Test Setup: Test vessels or bottles are filled with a suitable nitrification medium, which provides the necessary nutrients and environmental conditions for the growth and activity of nitrifying bacteria. Each vessel contains a specific concentration of the test substance, and multiple replicates are usually used for each concentration.
    • Incubation: The test vessels are incubated under controlled conditions, typically at a specific temperature and pH, to allow the nitrifying bacteria to grow and oxidize ammonia. The incubation period varies but is often around 3 to 6 hours.
    • Oxygen Consumption Measurement: The oxygen consumption or respiration rate of the nitrifying bacteria is measured using dissolved oxygen probes or sensors. The rate of oxygen consumption is an indicator of the nitrifying bacteria’s metabolic activity and serves as a measure of their health and vitality.
    • Data Analysis: The measured oxygen consumption rates for each concentration of the test substance are compared to the control (no test substance) to assess the level of inhibition. The concentration at which a significant decrease in oxygen consumption occurs indicates the toxicity threshold for the nitrifying bacteria.
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