Microbial Fermentation and Mammalian Cell Culture Analysis
The advantages of Measuring Oxygen Uptake Rate (OUR), Carbon Dioxide Evolution Rate (CER) and Respiration Quotient (RQ) have been utilized by Microbiologists for Aerobic Fermentation Applications for many years, its deployment to Cell Culture Applications is a relatively new development but it can yield important results.
Know Your Cells Activity in Seconds
OUR is often one of the best real-time indicators of biological activity. As viable cells grow oxygen consumption generally increases. CER provides a complementary metabolic measurement, CER measures how rapidly the culture generates CO₂ through metabolism.
The MGA 1200 CS Mass Spectrometer is an on-line analyzer that can measure up to 16 gases simultaneously in 0.1 second, making it one of the fastest process analyzers for OUR & CER measurements. This speed can be used to multiplex many samples (28) thus increasing its value to the end-user.
The MGA 1200 CS Mass Spectrometer can measure oxygen, carbon dioxide, and other gases such as nitrogen, argon, ammonia, ethanol, and methanol. Knowing these gases can assist in a complete characterization of cell growth. OUR can provide an indirect estimate of viable cell density (VCD) without having to repeatedly remove samples. This means you can potentially obtain metabolic information every few seconds or minutes, rather than waiting for laboratory analyses.
Gas Measurements of Small Bioreactors and Single Use Bioreactors (SUB) are now Possible
The extremely low flow rates (6 cc/min) and pressures (0.1 PSIG) for Small Bioreactors and SUB have made gas analysis very difficult in the past. There is the additional challenge of accurately detecting small changes in gas concentrations in these bioreactors.
Process Insights has developed a new sample system for the requirements of Cell Culture Applications. This sample system is designed to operate with SUB and other types of bioreactors as small as 250 mL and gas pressures as low as 0.1 PSIG.
The MGA 1200 CS Mass Spectrometer has the highest analytical precision available in any gas analyzer; this precision provides the ability to see small changes in bioreactor off-gas under these challenging conditions. The MGA has a 24-hour precision of 0.005% RSD and a two-week precision of 0.01% RSD. This stability makes it an excellent analyzer for long cell growth applications.
Process Insights Fixed Magnetic Sector Mass Spectrometer the MGA 1200 CS is the best choice for any cell culture facility; laboratory, pilot plant or full-scale production, with the ability to measure all sizes of Bioreactors from 3 L up to 10,000 L. The MGA 1200 CS combines high precision and low maintenance, with rapid measurements. Many Major Pharmaceutical Facilities exclusively use the MGA 1200 CS, contact us and arrange for a demonstration to see for yourself why they utilize the MGA 1200 CS for their critical cell culture monitoring and control.
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